Electron Microscope Fixative

In histopathology, tissue must be fixed in time and effectively before subsequent embedding and sectioning. The role of tissue fixation is to maintain the inherent morphology and structure of tissues and cells, prevent bacterial corrosion and the autolysis of tissue cells, preserve the inherent substances in cells, solidify the proteins in cells, minimize or terminate the reaction of endogenous and exogenous enzymes, to keep the cells or tissues essentially as they were at the time of life, and can also harden the tissues and facilitate the repair of tissues. For some infectious specimens, fixation can prevent the spread of disease; preserve the gross specimen. Finally, fixation also enhance staining. Glutaraldehyde is a commonly used fixative, which preserves the fine structure of cells well, but its permeability is weak, which is mainly used for the early fixation of electron microscope samples.